(D) Likewise, a comparison of your absorbance with the reference of 454 nm in opposition to the isosbestic point at 490 nm didn't show a rise in the extent of MIC formation after some time. Results from (A and B) depict the necessarily mean and S.D. of 3 independent experiments https://oss12816710987.blogkoo.com/the-smart-trick-of-pimpinellin-that-no-one-is-discussing-46028312